How to use the cutadapt.modifiers.QualityTrimmer function in cutadapt

def test_quality_trimmer():
    read = Sequence('read1', 'ACGTTTACGTA', '##456789###')

    qt = QualityTrimmer(10, 10, 33)
    assert qt(read, ModificationInfo(read)) == Sequence('read1', 'GTTTAC', '456789')

    qt = QualityTrimmer(0, 10, 33)
    assert qt(read, ModificationInfo(read)) == Sequence('read1', 'ACGTTTAC', '##456789')

    qt = QualityTrimmer(10, 0, 33)
    assert qt(read, ModificationInfo(read)) == Sequence('read1', 'GTTTACGTA', '456789###')

def test_quality_trimmer():
    read = Sequence('read1', 'ACGTTTACGTA', '##456789###')

    qt = QualityTrimmer(10, 10, 33)
    assert qt(read, ModificationInfo(read)) == Sequence('read1', 'GTTTAC', '456789')

    qt = QualityTrimmer(0, 10, 33)
    assert qt(read, ModificationInfo(read)) == Sequence('read1', 'ACGTTTAC', '##456789')

    qt = QualityTrimmer(10, 0, 33)
    assert qt(read, ModificationInfo(read)) == Sequence('read1', 'GTTTACGTA', '456789###')

def test_quality_trimmer():
    read = Sequence('read1', 'ACGTTTACGTA', '##456789###')

    qt = QualityTrimmer(10, 10, 33)
    assert qt(read, ModificationInfo(read)) == Sequence('read1', 'GTTTAC', '456789')

    qt = QualityTrimmer(0, 10, 33)
    assert qt(read, ModificationInfo(read)) == Sequence('read1', 'ACGTTTAC', '##456789')

    qt = QualityTrimmer(10, 0, 33)
    assert qt(read, ModificationInfo(read)) == Sequence('read1', 'GTTTACGTA', '456789###')

def __init__(self, queue=None, results=None, adapter=None, phred64=False):
		super(Worker, self).__init__()
		self.queue=queue
		self.results = results
		self.phred = 64 if phred64 else 33
		self.modifiers = [QualityTrimmer(0, 10, self.phred)]
		self.adapters = []
		self.error_rate = 0.12
		self.min_length = 16
		if adapter.startswith('+'):
			self.modifiers.append(UnconditionalCutter(int(adapter)))
		elif adapter == 'none':
			self.adapter = None
		else:
			self.adapters = parse_adapters(adapter, error_rate=self.error_rate)
			adapter_cutter = AdapterCutter(self.adapters)
			self.modifiers.append(adapter_cutter)

# When adapters are being trimmed only in R1 or R2, override the pair filter mode
    # as using the default of 'any' would regard all read pairs as untrimmed.
    if isinstance(pipeline, PairedEndPipeline) and (not adapters2 or not adapters) and (
            args.discard_untrimmed or args.untrimmed_output or args.untrimmed_paired_output):
        pipeline.override_untrimmed_pair_filter = True

    add_unconditional_cutters(pipeline, args.cut, args.cut2, paired)

    pipeline_add = pipeline.add_both if paired else pipeline.add

    if args.nextseq_trim is not None:
        pipeline_add(NextseqQualityTrimmer(args.nextseq_trim, args.quality_base))
    if args.quality_cutoff is not None:
        cutoffs = parse_cutoffs(args.quality_cutoff)
        pipeline_add(QualityTrimmer(cutoffs[0], cutoffs[1], args.quality_base))

    add_adapter_cutter(
        pipeline,
        adapters,
        adapters2,
        paired,
        args.pair_adapters,
        args.action,
        args.times,
        args.reverse_complement,
    )

    for modifier in modifiers_applying_to_both_ends_if_paired(args):
        pipeline_add(modifier)

    # Set filtering parameters