How to use the opentrons.labware.load function in opentrons

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github Opentrons / Protocols / protocols / 4c4650 / csv_dilution.ot2.py View on Github external
p1000_mount: StringSelection('right', 'left') = 'right',
        volume_CSV: FileInput = example_csv
):
    # pipette
    p1000 = instruments.P1000_Single(mount=p1000_mount, tip_racks=tipracks1000)

    # parse
    volumes = [
        float(line.split(',')[0]) for line in volume_CSV.splitlines() if line]
    if len(volumes) > 48*9:
        raise Exception('Too many volumes (' + str(len(volumes)) + 'for deck.')

    # load tube tube racks
    num_tuberacks = math.ceil(len(volumes)/48)
    tuberacks = [
        labware.load(rack_name, slot, 'destination tuberack ' + str(i+1))
        for i, slot in enumerate(range(4, 12))][:num_tuberacks]
    all_tubes = [tube for rack in tuberacks for tube in rack.wells()]

    tip1000_count = 0
    tip1000_max = len(tipracks1000)*96

    def pick_up():
        nonlocal tip1000_count
        if tip1000_count == tip1000_max:
            robot.pause('Refill 1000ul tipracks before resuming.')
            p1000.reset()
            tip1000_count = 0
        tip1000_count += 1
        p1000.pick_up_tip()

    # perform dilution transfer
github Opentrons / Protocols / protocols / 1506-gencell-pharma-part3 / labeling_mastermix_preparation.ot2.py View on Github external
def run_custom_protocol(
        sample_num: StringSelection('4', '8', '12', '16')='4'
        ):

    sample_num = int(sample_num)

    # labware setup
    eppendorf_rack = labware.load('opentrons-tuberack-2ml-eppendorf', '5')
    plate = labware.load('PCR-strip-tall', '2')
    tiprack_10 = labware.load('tiprack-10ul', '8')
    tipracks_300 = [labware.load('opentrons-tiprack-300ul', slot)
                    for slot in ['3', '6', '9']]

    # instruments setup
    p10 = instruments.P10_Single(
        mount='left',
        tip_racks=[tiprack_10])

    p50 = instruments.P50_Single(
        mount='right',
        tip_racks=tipracks_300)

    # reagent setup
    water = eppendorf_rack.wells('A1')
github Opentrons / Protocols / protocols / 1607 / cell_culture_assay.ot2.py View on Github external
depth=19,
        volume=3600
    )

# load labware and modules
tempmod_1 = modules.load('tempdeck', '10')
temp_plate1 = labware.load(plate_name, '10', 'plate 1', share=True)
tempmod_2 = modules.load('tempdeck', '7')
temp_plate2 = labware.load(plate_name, '7', 'plate 2', share=True)
tempmod_3 = modules.load('tempdeck', '4')
temp_plate3 = labware.load(plate_name, '4', 'plate 3', share=True)
tempmod_4 = modules.load('tempdeck', '6')
temp_plate4 = labware.load(plate_name, '6', 'plate 4', share=True)
tips1000 = [labware.load('tiprack-1000ul', slot) for slot in ['5', '11']]
tubes = labware.load('opentrons-tuberack-15_50ml', '8')
waste = labware.load('point', '1')

tempmod1.set_temperature(37)
tempmod2.set_temperature(37)
tempmod3.set_temperature(37)
tempmod4.set_temperature(37)
tempmod4.wait_for_temp()

# reagents
media = [tubes.wells(well) for well in ['A3', 'A4']]


def run_custom_protocol(
    pipette_mount: StringSelection('right', 'left') = 'right'
):

    # height track function
github Opentrons / Protocols / protocols / 821-john-b-pierce-laboratory / Mag-Bind_Ultra_Pure_Plasmid_Kit.ot2.py View on Github external
flat_plate = labware.load('96-flat', '5')

# reagent setup
RNase_A = trough.wells('A1')
solution_II = trough.wells('A2')
N3_buffer = trough.wells('A3')
LC_beads = trough.wells('A4')
ETR_bind_buffer = trough.wells('A5')
Mag_Bind_RQ = trough.wells('A6')
ETR_wash_buffer = trough.wells('A7')
VHB_buffer = trough.wells('A8')
SPM_wash_buffer = trough.wells('A9')
elution_buffer = trough.wells('A10')

# tipracks
tipracks300 = [labware.load('tiprack-200ul', slot)
               for slot in ['6', '7', '8', '9', '10']]

tiprack50 = labware.load('tiprack-200ul', '11')

# pipette setup
m300 = instruments.P300_Multi(
    mount='left',
    tip_racks=tipracks300)

m50 = instruments.P50_Multi(
    mount='right',
    tip_racks=[tiprack50])


def run_custom_protocol(
        elution_buffer_volume: float=50):
github Opentrons / Protocols / protocols / 1611 / elisa.ot2.py View on Github external
if elisa_plate_name not in labware.list():
    labware.create(
        elisa_plate_name,
        grid=(12, 8),
        spacing=(9, 9),
        diameter=7,
        depth=11.3,
        volume=350
    )

# load labware
elisa_plates = [
    labware.load(elisa_plate_name, str(slot), 'elisa plate ' + str(slot))
    for slot in range(1, 9)
]
dilution_plate = labware.load(
    deep_plate_name, '9', 'dilution plate with tubes')
accessory_tuberack = labware.load(deep_plate_name, '10', 'accessory tuberack')
tips300 = labware.load('opentrons-tiprack-300ul', '11')

# tube setup
accessory_tubes = [tube for tube in accessory_tuberack.columns('1')]


def run_custom_protocol(
        p300_single_mount: StringSelection('left', 'right') = 'left',
        p300_multi_mount: StringSelection('right', 'left') = 'right'
):
    # check
    if p300_multi_mount == p300_single_mount:
        raise Exception('Input different mount sides for pipettes.')
github Opentrons / Protocols / protocols / 1503-dr-travis-glenn-lab-uga-ehs-part1 / NGS_library_prep_part1.ot2.py View on Github external
'phenix-filter-tiprack-300ul')='phenix-filter-tiprack-300ul'):

    for tiprack_type in [p10_tiprack_type, p50_tiprack_type]:
        if tiprack_type not in labware.list():
            labware.create(
                tiprack_type,
                grid=(12, 8),
                spacing=(9, 9),
                diameter=5,
                depth=60)

    # labware setup
    plate = labware.load('biorad-hardshell-96-PCR', '1')
    mag_module = modules.load('magdeck', '4')
    mag_plate = labware.load('biorad-hardshell-96-PCR', '4', share=True)
    reagent_tubes = labware.load('96-PCR-tall', '5')
    deep_plate = labware.load('96-deep-well', '7')
    trough = labware.load('trough-12row', '8')

    tipracks_10 = [labware.load(p10_tiprack_type, slot)
                   for slot in ['10', '11']]
    tipracks_50 = [labware.load(p50_tiprack_type, slot)
                   for slot in ['2', '3', '6', '9']]

    # instruments setup
    m10 = instruments.P10_Multi(
        mount='right',
        tip_racks=tipracks_10)
    m50 = instruments.P50_Multi(
        mount='left',
        tip_racks=tipracks_50)
github Opentrons / Protocols / protocols / qiaseq-pt-4 / pt4_target_enrichment.ot2.py View on Github external
from opentrons import labware, instruments, robot
from otcustomizers import StringSelection
import math

metadata = {
    'protocolName': 'QIAseq Targeted DNA Panel for Illumina Instruments Part 4:\
 Target enrichment',
    'author': 'Nick ',
    'source': 'Custom Protocol Request'
}

# load modules and labware
rxn_plate = labware.load(
    'opentrons_96_aluminumblock_biorad_wellplate_200ul',
    '1',
    'reaction plate'
)
reagent_tuberack = labware.load(
    'opentrons_24_aluminumblock_generic_2ml_screwcap',
    '2',
    'reagent tuberack (pre-chilled)'
)
strips = labware.load(
    'opentrons_96_aluminumblock_generic_pcr_strip_200ul', '3', 'strips')
tips50_single = labware.load('opentrons_96_tiprack_300ul', '4')
tips_50_multi = [
    labware.load('opentrons_96_tiprack_300ul', slot) for slot in ['5', '6']
]
github Opentrons / Protocols / protocols / illumina_16s_prep / illumina_16s_prep.ot2.py View on Github external
from opentrons import labware, instruments

bead_plate = labware.load('96-PCR-flat', '4', 'bead_plate')
spin_plate = labware.load('96-PCR-flat', '5', 'spin_plate')
collection1mL_1 = labware.load('96-PCR-flat', '6', 'collection1mL_1')
collection1mL_2 = labware.load('96-PCR-flat', '7', 'collection1mL_2')
collection1mL_3 = labware.load('96-PCR-flat', '8', 'collection1mL_3')
collection1mL_4 = labware.load('96-PCR-flat', '9', 'collection1mL_4')
collection2mL_1 = labware.load('96-PCR-flat', '10', 'collection2mL_1')

trough = labware.load('trough-12row', '11', 'trough')

m300rack1 = labware.load('tiprack-200ul', '1', 'm300rack1')
m300rack2 = labware.load('tiprack-200ul', '2', 'm300rack2')
m300rack3 = labware.load('tiprack-200ul', '3', 'm300rack3')

m300 = instruments.P300_Multi(
    tip_racks=[m300rack1, m300rack2, m300rack3],
    mount='left',
)

ethanol = trough.cols('1')
bead_solution = trough.cols('2')
C1 = trough.cols('3')
github Opentrons / Protocols / protocols / 1348-harvard-university / scheduled_reactant_addition.ot2.py View on Github external
from opentrons import labware, instruments

# generate custom labware
custom_6_well_plate = 'custom-6-well-plate'
if custom_6_well_plate not in labware.list():
    labware.create(
        custom_6_well_plate,
        grid=(3, 2),
        spacing=(40, 38),
        diameter=34.9,
        depth=65)

# labware setup
stock = labware.load(custom_6_well_plate, '1')
reactor = labware.load(custom_6_well_plate, '2')

tipracks_300 = [labware.load('opentrons-tiprack-300ul', slot)
                for slot in ['4', '5']]


# pipette setup
p300 = instruments.P300_Single(
    mount='right',
    tip_racks=tipracks_300)


for index, reactor in enumerate(reactor.wells()[:3]):
    if index < 2:
        time = 2
    else:
github Opentrons / Protocols / protocols / 1f9218 / capsule_filling.ot2.py View on Github external
def run_custom_protocol(
        p1000_mount: StringSelection('right', 'left') = 'right',
        number_of_capsules_to_fill: int = 100,
        volume_to_fill_in_ul: float = 200
):
    # check
    if volume_to_fill_in_ul < 100:
        raise Exception('Invalid volume for P1000 pipette')

    # load capsule plates
    slots = ['4', '10'] if number_of_capsules_to_fill > 100 else ['4']
    capsule_racks = [
        labware.load(capsule_rack_name, slot, 'capsules') for slot in slots]

    # pipette
    p1000 = instruments.P1000_Single(mount=p1000_mount, tip_racks=[tips1000])
    p1000.set_flow_rate(aspirate=100)

    max_vol = oil.properties['total-liquid-volume']*0.9
    total_vol = 0

    x = 108.00
    y = 72.00
    h = -10

    def height_track():
        nonlocal h
        dh = (volume_to_fill_in_ul/(x*y))+0.01
        h = h - dh if h - dh > -37 else -37