How to use the opentrons.instruments.P300_Single function in opentrons

def run_custom_protocol(
    pipette_type: StringSelection(
        'p300-Single', 'p300-Multi', 'p50-Single', 'p50-Multi')='p300-Multi',
    dilution_factor: float=1.5,
    num_of_dilutions: int=10,
    final_volume: float=200.0,
    tip_reuse_strategy: StringSelection(
        'reuse one tip', 'new tip each time')='reuse one tip'):

    pip_name = pipette_type.split('-')
    print(pip_name[1])

    if pipette_type == 'p300-Single':
        pipette = instruments.P300_Single(
            mount='left',
            tip_racks=[tiprack])
    elif pipette_type == 'p50-Single':
        pipette = instruments.P50_Single(
            mount='left',
            tip_racks=[tiprack])
    elif pipette_type == 'p300-Multi':
        pipette = instruments.P300_Multi(
            mount='left',
            tip_racks=[tiprack])
    elif pipette_type == 'p50-Multi':
        pipette = instruments.P50_Multi(
            mount='left',
            tip_racks=[tiprack])

    new_tip = 'never' if tip_reuse_strategy == 'reuse one tip' else 'always'

def run_custom_protocol(
        volume_csv: FileInput=csv_example,
        dna_volume: float=2):

    # labware setup
    tuberack = labware.load('trough-12row', '2')
    plate = labware.load('optical-96-well', '3', 'Output Plate')
    dna = labware.load('optical-96-well', '6', 'DNA Plate')

    tiprack_10 = labware.load('tiprack-10ul', '5')
    tiprack_300 = labware.load('opentrons-tiprack-300ul', '1')

    # instruments setup
    p300 = instruments.P300_Single(
        mount='left',
        tip_racks=[tiprack_300])

    m10 = instruments.P10_Multi(
        mount='right',
        tip_racks=[tiprack_10])

    # reagent setup
    buff = tuberack.wells('A1')

    dest_wells, volumes, col_num = csv_to_list(volume_csv)

    # transfer buffer
    buff_volume_tracker = buff.max_volume()
    p300.pick_up_tip()
    for dest, vol in zip(dest_wells, volumes):

trough = labware.load('tube-rack-15_50ml', '9')

P1 = labware.load('96-flat', '1')  # Chemicals
P2 = labware.load('96-flat', '2')  # Plate taking in chemical CP1
P3 = labware.load('96-deep-well', '3')  # Samples
P4 = labware.load('96-flat', '4')  # Plate taking in chemical CP2
P5 = labware.load('96-flat', '5')  # Plate taking in checmial CP3
P6 = labware.load('96-flat', '6')  # Plate taking in chemical CP4

# pipette setup
m50 = instruments.P50_Multi(
    mount='left',
    tip_racks=tipracks)

p300 = instruments.P300_Single(
    mount='right',
    tip_racks=tipracks)


targets = [P2, P4, P5, P6]

# Transfer media to all of the target plates
p300.pick_up_tip()
for target_plate in targets:
    for col_num in range(1, 11):
        p300.transfer(182, trough['A1'], target_plate.cols(col_num)[1:7],
                      new_tip='never')
p300.drop_tip()

# Add destinations of chemical 1(x), 2(x+1), 3(x+2), 4(x)...12(x+2)
target_list = []

def run_custom_protocol(
        p300_single_mount: StringSelection('left', 'right') = 'left',
        p300_multi_mount: StringSelection('right', 'left') = 'right'
):
    # check
    if p300_multi_mount == p300_single_mount:
        raise Exception('Input different mount sides for pipettes.')

    # pipettes
    p300 = instruments.P300_Single(
        mount=p300_single_mount, tip_racks=[tips300])
    m300 = instruments.P300_Multi(
        mount=p300_multi_mount, tip_racks=[tips300])

    # perform dilution on deepwell plate
    m300.pick_up_tip()
    for source, dest in zip(
            dilution_plate.rows('A')[0:11], dilution_plate.rows('A')[1:12]):
        m300.transfer(450, source, dest.top(), new_tip='never')
        m300.blow_out(dest.top())
        m300.mix(5, 200, dest)
        m300.blow_out(dest.top())

    # transfer last dilution to accessory tubes in first column of rack in
    # slot 10
    m300.transfer(

]
        ]
        pass

    if p300_type == 'multi':
        # holder
        pip300 = instruments.P300_Multi(mount=p300_mount)
        [samples300, eluates] = [
            plate.rows('A')[:num_cols] for plate in [
                deep_plate, elution_plate
            ]
        ]
        pass
    else:
        # holder
        pip300 = instruments.P300_Single(mount=p300_mount)
        [samples300, eluates] = [
            plate.wells()[:number_of_samples_to_process] for plate in [
                deep_plate, elution_plate
            ]
        ]
        pass

    if magdeck._engaged:
        magdeck.disengage()

    pip50.pick_up_tip(tips_1x.wells('A1'))

    # transfer 20ul of proteinase k to each well
    pip50vol = 0
    for well in samples50:
        if pip50vol == 0:

elif capacity == 'p50' or capacity == 'p300':
        tiprack_name = 'opentrons_96_tiprack_300ul'
    else:
        tiprack_name = 'opentrons_96_tiprack_1000ul'

    if pipette_type == 'p1000_Single':
        tipracks = [labware.load('opentrons_96_tiprack_1000ul', slot)
                    for slot in slots]
        pipette = instruments.P1000_Single(
            mount=pipette_axis,
            tip_racks=tipracks)

    elif pipette_type == 'p300_Single':
        tipracks = [labware.load('opentrons_96_tiprack_300ul', slot)
                    for slot in slots]
        pipette = instruments.P300_Single(
            mount=pipette_axis,
            tip_racks=tipracks)

    elif pipette_type == 'p50_Single':
        tipracks = [labware.load('opentrons_96_tiprack_300ul', slot)
                    for slot in slots]
        pipette = instruments.P50_Single(
            mount=pipette_axis,
            tip_racks=tipracks)

    elif pipette_type == 'p10_Single':
        tipracks = [labware.load('opentrons_96_tiprack_10ul ', slot)
                    for slot in slots]
        pipette = instruments.P10_Single(
            mount=pipette_axis,
            tip_racks=tipracks)

# labware setup
trough = labware.load(trough_name, '1')
plate_1 = labware.load('96-flat', '2')
output_1 = labware.load('96-flat', '3')
plate_2 = labware.load('96-flat', '5')
output_2 = labware.load('96-flat', '6')
tipracks_s = [labware.load('opentrons-tiprack-300ul', slot)
              for slot in ['4', '8']]
tipracks_m = [labware.load('opentrons-tiprack-300ul', slot)
              for slot in ['7', '9']]

# reagent setup
buffer = trough.wells('A1')

# instruments setup
p300 = instruments.P300_Single(
    mount='left',
    tip_racks=tipracks_s)
m300 = instruments.P300_Multi(
    mount='right',
    tip_racks=tipracks_m)

example = """
Plate,Well,Volume
1,A1,
1,A2,245
1,A3,248
1,A4,262
1,A5,300
1,A6,287
1,A7,283
1,A8,259

else:
        stock_plate = labware.load(
            'corning_96_wellplate_360ul_flat', '1', 'stock plate')
    test_plates = [
        labware.load(
            'corning_96_wellplate_360ul_flat',
            str(slot),
            'test plate ' + str(i)
        )
        for i, slot in enumerate(
            ['3', '6', '7', '8', '9', '10', '11'][:number_of_test_plates])
    ]

    # pipettes
    p50 = instruments.P50_Single(mount=p50_single_mount, tip_racks=[tips50])
    p300 = instruments.P300_Single(
        mount=p300_single_mount, tip_racks=[tips300])

    tubes = {
        'growth medium': [gm, 100, 14000],
        'water': [water, 100, 14000]
    }
    r15 = 14.90/2
    min_h = 10

    def h_track(vol, tube):
        nonlocal tubes
        # calculate aspiration height
        tubes[tube][2] -= vol
        if tubes[tube][2] < 1000:
            robot.pause(
                'Fill liquid in ' + tube + ' tube (rack well '

tiprack_200 = labware.load('tiprack-200ul', '3')
tuberack_1 = labware.load('opentrons-tuberack-2ml-screwcap', '4')
tuberack_2 = labware.load('opentrons-tuberack-2ml-screwcap', '5')
plates = [labware.load('96-deep-well', slot)
          for slot in ['6', '7', '8', '9', '10', '11']]

plates_name = ['plate '+i for i in ['1', '2', '3', '7', '10', '11']]
source = {'tube rack 1': tuberack_1, 'tube rack 2': tuberack_2}
source.update({name: plate for name, plate in zip(plates_name, plates)})

# instrument setup
p10 = instruments.P10_Single(
    mount='right',
    tip_racks=tipracks_10)

p300 = instruments.P300_Single(
    mount='left',
    tip_racks=[tiprack_200])

# reagent setup
sample_1 = tuberack_1.wells('A6')
sample_2 = tuberack_1.wells('B6')
sample_3 = tuberack_1.wells('C6')
sample_4 = tuberack_1.wells('D6')

p10_tip_count = 0
p300_tip_count = 0


example_1 = """
Tube Rack Name,Tube Position ,Sample Name,Volume (uL)
Tube Rack 2,A01,Water,282