How to use the opentrons.instruments.P50_Single function in opentrons

if 'tube-rack-5x12' not in labware.list():
    labware.create(
        'tube-rack-5x12',
        grid=(12, 5),
        spacing=(20, 20),
        diameter=16,
        depth=90)

# labware setup
trough1 = labware.load('trough-12row', '7')
sample_rack = labware.load('tube-rack-5x12', '4')
tiprack_50 = labware.load('tiprack-200ul', '8')
tiprack_1000 = labware.load('tiprack-1000ul', '9')

# pipette setup
p50 = instruments.P50_Single(
    mount='left',
    tip_racks=[tiprack_50]
    )

p1000 = instruments.P1000_Single(
    mount='right',
    tip_racks=[tiprack_1000]
    )


def run_custom_protocol(
    number_of_samples: int=10
        ):

    # number of racks for serial dilutions
    rack_num = number_of_samples // 12 + (

from opentrons import labware, instruments
from otcustomizers import FileInput, StringSelection

# labware setup
tuberack = labware.load('tube-rack-2ml', '2')

tiprack = labware.load('tiprack-200ul', '4')

# pipette setup
p50 = instruments.P50_Single(
    mount='left',
    tip_racks=[tiprack]
    )


def well_csv_to_list(csv_string):
    """
    Takes a csv string and flattens it to a list
    """
    return [
        cell
        for line in (csv_string.split('\n')) if line.strip()
        for cell in line.split(',') if cell
    ]

)

# labware setup
reagent_list = {str(slot): labware.load(rack_name, str(slot))
                for slot, rack_name in enumerate(rack_names, 1)}

tipracks_10 = [labware.load('tiprack-10ul', slot)
               for slot in ['10', '11']]
tipracks_50 = [labware.load('opentrons-tiprack-300ul', slot)
               for slot in ['7', '8']]

# instruments setup
p10 = instruments.P10_Single(
    mount='left',
    tip_racks=tipracks_10)
p50 = instruments.P50_Single(
    mount='right',
    tip_racks=tipracks_50)


def run_custom_protocol(
        transfer_csv: FileInput=transfer_csv_example,
        plate_type: StringSelection(
            'opentrons-aluminum-block-96-PCR-plate',
            'opentrons-aluminum-block-PCR-strips-200ul'
            )='opentrons-aluminum-block-96-PCR-plate'):

    def get_transfer_info(csv_string, reagent_list):
        info_list = [cell for line in csv_string.splitlines() if line
                     for cell in [line.split(',')]]
        sources = []
        volumes = []

flat_plate_name,
        grid=(12, 8),
        spacing=(9, 9),
        diameter=6.3,
        depth=10.8,
        volume=350
    )

# load labware
trough = labware.load('trough-12row', '1')
DNA_plate = labware.load(PCR_plate_name, '2')
destination_plate = labware.load(flat_plate_name, '3')
tips50 = [labware.load('opentrons-tiprack-300ul', slot) for slot in ['4', '5']]

# pipettes
p50 = instruments.P50_Single(mount='right', tip_racks=tips50)

# well setup
H2O = trough.wells('A7')
DNA_sources = [well for row in DNA_plate.rows() for well in row]
destination_wells = [well for row in destination_plate.rows() for well in row]


def extract_csv_transfers(file_string):
    # take in all rows of the file
    whole_file = file_string.splitlines()

    # pick off water transfers
    H2O_rows = whole_file[2:10]
    H2O_transfers = []
    for row in H2O_rows:
        els = row.split(',')

from opentrons import labware, instruments

metadata = {
    'protocolName': 'Drug Distribution in Triplicate',
    'author': 'Alise ',
    'source': 'Custom Protocol Request'
    }

# labware setup
tuberack = labware.load('opentrons-tuberack-2ml-eppendorf', '5')
plate = labware.load('96-flat', '2')
tiprack = labware.load('opentrons-tiprack-300ul', '4')

# instruments setup
p50 = instruments.P50_Single(
    mount='left',
    tip_racks=[tiprack])

drugs = [well for row in tuberack.rows() for well in row]

dests = [row(index, length=3)
         for row in plate.rows() for index in range(0, 7, 3)]


def run_custom_protocol(transfer_volume: float=20):
    for source, dest in zip(drugs, dests):
        p50.distribute(20, source, dest, blow_out=source)

'opentrons_24_tuberack_eppendorf_1.5ml_safelock_snapcap', '6')

# modules
tempdeck = modules.load('tempdeck', '5')
plate = labware.load(
    'opentrons_96_aluminumblock_biorad_wellplate_200ul', '5', share=True)
if not robot.is_simulating():
    tempdeck.set_temperature(4)
    tempdeck.wait_for_temp()

# pipettes
p10 = instruments.P10_Single(
    mount='right',
    tip_racks=[tips10]
)
p50 = instruments.P50_Single(
    mount='left',
    tip_racks=[tips50]
)


def run_custom_protocol(number_of_DNA_samples: int = 21,
                        number_of_oligo_standards: int = 8):
    # check invalid parameters
    if number_of_DNA_samples + number_of_oligo_standards > 30:
        raise Exception('Too many samples and standards for one plate.')

    # master mix setup
    master_mix = tubes.wells('A1')

    # DNA sample sources setup
    DNA_samples = tubes.wells('B1', length=number_of_DNA_samples)

from opentrons import labware, instruments
from otcustomizers import StringSelection

# labware setup
trough = labware.load('trough-12row', '1')
plate = labware.load('96-flat', '2')
tiprack = labware.load('opentrons-tiprack-300ul', '5')


# pipette setup
m300 = instruments.P300_Multi(
    mount='right',
    tip_racks=[tiprack])

p50 = instruments.P50_Single(
    mount='left',
    tip_racks=[tiprack])

# reagent setup
buffer = trough.wells('A1')


def run_custom_protocol(
        sample_container: StringSelection(
            'opentrons-tuberack-2ml-eppendorf',
            'opentrons-tuberack-2ml-screwcap',
            'opentrons-tuberack-15ml',
            'trough-12row')='opentrons-tuberack-2ml-eppendorf'):

    sample_container = labware.load(sample_container, '3')

plate_384 = labware.load('384-plate', '2')
tuberack = labware.load('tube-rack-2ml', '3')
source_tubes = tuberack.wells(0, length=8)

tiprack_10ul = labware.load('tiprack-10ul', '5')
tiprack_300ul = labware.load('opentrons-tiprack-300ul', '4')
tiprack2_300ul = labware.load('opentrons-tiprack-300ul', '6')
tiprack3_300ul = labware.load('opentrons-tiprack-300ul', '7')

# pipette setup
m10 = instruments.P10_Multi(
    mount='right',
    tip_racks=[tiprack_10ul]
    )

p50 = instruments.P50_Single(
    mount='left',
    tip_racks=[tiprack_300ul, tiprack2_300ul, tiprack3_300ul]
    )

# transfer PCR strip #1 to rows A, C, E, G, I, K, M, O
# transfer PCR strip #2 to rows B, D, F, H, J, L, N, P
m10.transfer(3, plate_96.cols('1'), [well for well in plate_384.rows('A')])
m10.transfer(3, plate_96.cols('2'), [well for well in plate_384.rows('B')])


# each source tube is distributed to 3 different columns in the plate
# distribute 7 uL to 6 wells every time it aspirates from the source
for index, each_tube in enumerate(source_tubes):
    p50.pick_up_tip()
    for row in plate_384.rows():
        for well in row.wells(index*3, length=3):

from opentrons import instruments, labware, robot

# labware definitions
tips_1000 = labware.load('tiprack-1000ul', '1')
samples = labware.load('tube-rack-2ml', '2')
output = labware.load('96-PCR-flat', '3')
tips_50 = labware.load('tiprack-200ul', '4')
trough = labware.load('trough-12row', '5')
deepwell = labware.load('96-deep-well', '6')

# instrument setup
p50 = instruments.P50_Single(
    tip_racks=[tips_50],
    mount='left')

p1000 = instruments.P1000_Single(
    tip_racks=[tips_1000],
    mount='right')

# reagents and variables setup
# setup for samples + initial two dilutions of 1:100 and 1:2500 in 2ml tuberack
buff = trough.wells('A1')  # location of buffer in trough
sam1 = samples.wells('A1')  # location of concentrated sample 1
sam2 = samples.wells('B1')  # location of concetrated sample 2
s1_dil1 = samples.wells('A2')  # location of 1:100 dilution of sample 1
s2_dil1 = samples.wells('B2')  # location of 1:100 dilution of sample 2
s1_dil2 = samples.wells('A3')  # location of 1:2500 dilution of sample 1
s2_dil2 = samples.wells('B3')  # location of 1:2500 dilution of sample 2