How to use the plantcv.roi_objects function in plantcv

# Identify objects
  device, id_objects3,obj_hierarchy3 = pcv.find_objects(masked2, ab_fill, device, args.debug)

  # Define ROI
  device, roi3, roi_hierarchy3= pcv.define_roi(masked2,'rectangle', device, None, 'default', args.debug,True, 650, 0,-450,-300)
 
  # Decide which objects to keep and combine with objects overlapping with black bars
  device,roi_objects3, hierarchy3, kept_mask3, obj_area1 = pcv.roi_objects(img,'cutto',roi3,roi_hierarchy3,id_objects3,obj_hierarchy3,device, args.debug)
  device, kept_mask4_1 = pcv.logical_or(ab_cnt3, kept_mask3, device, args.debug)
  device, kept_cnt = pcv.logical_or(ab_cnt3, kept_mask3, device, args.debug)
  device, kept_mask4 = pcv.fill(kept_mask4_1, kept_cnt, 200, device, args.debug)
  device, masked5 = pcv.apply_mask(masked2, kept_mask4, 'white', device, args.debug)
  device, id_objects4,obj_hierarchy4 = pcv.find_objects(masked5, kept_mask4, device, args.debug)
  device, roi4, roi_hierarchy4= pcv.define_roi(masked2,'rectangle', device, None, 'default', args.debug,False, 0, 0,0,0)
  device,roi_objects4, hierarchy4, kept_mask4, obj_area = pcv.roi_objects(img,'partial',roi4,roi_hierarchy4,id_objects4,obj_hierarchy4,device, args.debug)

 # Object combine kept objects
  device, obj, mask = pcv.object_composition(img, roi_objects4, hierarchy4, device, args.debug)
  
############## Analysis ################  
  
  # Find shape properties, output shape image (optional)
  device, shape_header,shape_data,shape_img = pcv.analyze_object(img, args.image, obj, mask, device,args.debug,args.outdir+'/'+filename)
   
  # Shape properties relative to user boundary line (optional)
  device, boundary_header,boundary_data, boundary_img1= pcv.analyze_bound(img, args.image,obj, mask, 375, device,args.debug,args.outdir+'/'+filename)
  
  # Determine color properties: Histograms, Color Slices and Pseudocolored Images, output color analyzed images (optional)
  device, color_header,color_data,norm_slice= pcv.analyze_color(img, args.image, kept_mask4, 256, device, args.debug,'all','rgb','v','img',300,args.outdir+'/'+filename)
  
  # Output shape and color data

device, masked2_a = pcv.rgb2gray_lab(masked2, 'a', device, args.debug)
  device, masked2_b = pcv.rgb2gray_lab(masked2, 'b', device, args.debug)
  
  # Threshold the green-magenta and blue images
  device, masked2a_thresh = pcv.binary_threshold(masked2_a, 127, 255, 'dark', device, args.debug)
  device, masked2b_thresh = pcv.binary_threshold(masked2_b, 128, 255, 'light', device, args.debug)
  device, ab_fill = pcv.logical_or(masked2a_thresh, masked2b_thresh, device, args.debug)
  
  # Identify objects
  device, id_objects,obj_hierarchy = pcv.find_objects(masked2, ab_fill, device, args.debug)

  # Define ROI
  device, roi1, roi_hierarchy= pcv.define_roi(masked2,'rectangle', device, None, 'default', args.debug,True, 550, 0,-600,-915)
  
  # Decide which objects to keep
  device,roi_objects, hierarchy3, kept_mask, obj_area = pcv.roi_objects(img,'partial',roi1,roi_hierarchy,id_objects,obj_hierarchy,device, args.debug)
  
  # Object combine kept objects
  device, obj, mask = pcv.object_composition(img, roi_objects, hierarchy3, device, args.debug)
  
############### Analysis ################  
  
  # Find shape properties, output shape image (optional)
  device, shape_header,shape_data,shape_img = pcv.analyze_object(img, args.image, obj, mask, device,args.debug,args.outdir+'/'+filename)
    
  # Shape properties relative to user boundary line (optional)
  device, boundary_header,boundary_data, boundary_img1= pcv.analyze_bound(img, args.image,obj, mask, 885, device,args.debug,args.outdir+'/'+filename)
  
  # Determine color properties: Histograms, Color Slices and Pseudocolored Images, output color analyzed images (optional)
  device, color_header,color_data,color_img= pcv.analyze_color(img, args.image, kept_mask, 256, device, args.debug,None,'v','img',300,args.outdir+'/'+filename)
  
  # Output shape and color data

# Median Filter
  device, soil_mblur = pcv.median_blur(soil_fill, 5, device, args.debug)
  device, soil_cnt = pcv.median_blur(soil_fill, 5, device, args.debug)
  
  # Apply mask (for vis images, mask_color=white)
  device, masked2 = pcv.apply_mask(soil_masked, soil_cnt, 'white', device, args.debug)
  
  # Identify objects
  device, id_objects,obj_hierarchy = pcv.find_objects(masked2, soil_cnt, device, args.debug)

  # Define ROI
  device, roi1, roi_hierarchy= pcv.define_roi(img,'circle', device, None, 'default', args.debug,True, 0,0,-50,-50)
  
  # Decide which objects to keep
  device,roi_objects, hierarchy3, kept_mask, obj_area = pcv.roi_objects(img,'partial',roi1,roi_hierarchy,id_objects,obj_hierarchy,device, args.debug)
  
  # Object combine kept objects
  device, obj, mask = pcv.object_composition(img, roi_objects, hierarchy3, device, args.debug)
  
############## Analysis ################  
  
  # Find shape properties, output shape image (optional)
  device, shape_header,shape_data,shape_img = pcv.analyze_object(img, args.image, obj, mask, device,args.debug,args.outdir+'/'+filename)
  
  # Determine color properties: Histograms, Color Slices and Pseudocolored Images, output color analyzed images (optional)
  device, color_header,color_data,norm_slice= pcv.analyze_color(img, args.image, kept_mask, 256, device, args.debug,'all','rgb','v','img',300,args.outdir+'/'+filename)
  
  # Output shape and color data
  pcv.print_results(args.image, shape_header, shape_data)
  pcv.print_results(args.image, color_header, color_data)

device, maskedb_thresh2 = pcv.binary_threshold(masked_b2, 170, 255, 'light', device, args.debug)
  device, ab2 = pcv.logical_and(maskeda_thresh2, maskedb_thresh2, device, args.debug)
  device, ab_cnt2 = pcv.logical_or(maskeda_thresh2, maskedb_thresh2, device, args.debug)
  device, ab_fill2 = pcv.fill(ab2, ab_cnt2, 1500, device, args.debug)
  
  device, ab_cnt3 = pcv.logical_or(ab_fill1, ab_fill2, device, args.debug)
  device, masked3 = pcv.apply_mask(masked2, ab_cnt3, 'white', device, args.debug)
  
  # Identify objects
  device, id_objects3,obj_hierarchy3 = pcv.find_objects(masked2, ab_fill, device, args.debug)

  # Define ROI
  device, roi3, roi_hierarchy3= pcv.define_roi(masked2,'rectangle', device, None, 'default', args.debug,True, 500, 0,-450,-750)
 
  # Decide which objects to keep and combine with objects overlapping with black bars
  device,roi_objects3, hierarchy3, kept_mask3, obj_area1 = pcv.roi_objects(img,'cutto',roi3,roi_hierarchy3,id_objects3,obj_hierarchy3,device, args.debug)
  device, kept_mask4_1 = pcv.logical_or(ab_cnt3, kept_mask3, device, args.debug)
  device, kept_cnt = pcv.logical_or(ab_cnt3, kept_mask3, device, args.debug)
  device, kept_mask4 = pcv.fill(kept_mask4_1, kept_cnt, 12, device, args.debug)
  device, masked5 = pcv.apply_mask(masked2, kept_mask4, 'white', device, args.debug)
  device, id_objects4,obj_hierarchy4 = pcv.find_objects(masked5, kept_mask4, device, args.debug)
  device, roi4, roi_hierarchy4= pcv.define_roi(masked2,'rectangle', device, None, 'default', args.debug,False, 0, 0,0,0)
  device,roi_objects4, hierarchy4, kept_mask4, obj_area = pcv.roi_objects(img,'partial',roi4,roi_hierarchy4,id_objects4,obj_hierarchy4,device, args.debug)

 # Object combine kept objects
  device, obj, mask = pcv.object_composition(img, roi_objects4, hierarchy4, device, args.debug)
  
############## Analysis ################  
  
  # Find shape properties, output shape image (optional)
  device, shape_header,shape_data,shape_img = pcv.analyze_object(img, args.image, obj, mask, device,args.debug,args.outdir+'/'+filename)

device, masked2_a = pcv.rgb2gray_lab(masked2, 'a', device, args.debug)
  device, masked2_b = pcv.rgb2gray_lab(masked2, 'b', device, args.debug)
  
  # Threshold the green-magenta and blue images
  device, masked2a_thresh = pcv.binary_threshold(masked2_a, 127, 255, 'dark', device, args.debug)
  device, masked2b_thresh = pcv.binary_threshold(masked2_b, 128, 255, 'light', device, args.debug)
  device, ab_fill = pcv.logical_or(masked2a_thresh, masked2b_thresh, device, args.debug)
  
  # Identify objects
  device, id_objects,obj_hierarchy = pcv.find_objects(masked2, ab_fill, device, args.debug)
  
  # Define ROI
  device, roi1, roi_hierarchy= pcv.define_roi(masked2,'rectangle', device, None, 'default', args.debug,True, 550, 0,-600,-915)
  
  # Decide which objects to keep
  device,roi_objects, hierarchy3, kept_mask, obj_area = pcv.roi_objects(img,'partial',roi1,roi_hierarchy,id_objects,obj_hierarchy,device, args.debug)
  
  # Object combine kept objects
  device, obj, mask = pcv.object_composition(img, roi_objects, hierarchy3, device, args.debug)
  
  ############## VIS Analysis ################
  
  outfile=False
  if args.writeimg==True:
    outfile=args.outdir+"/"+filename
  
  # Find shape properties, output shape image (optional)
  device, shape_header,shape_data,shape_img = pcv.analyze_object(img, args.image, obj, mask, device,args.debug,outfile)
  
  # Shape properties relative to user boundary line (optional)
  device, boundary_header,boundary_data, boundary_img1= pcv.analyze_bound(img, args.image,obj, mask, 885, device,args.debug,outfile)

device, ab_cnt = pcv.logical_or(maskeda_thresh, maskedb_thresh, device, args.debug)
  
  # Fill small objects
  device, ab_fill = pcv.fill(ab, ab_cnt, 20, device, args.debug)
  
  # Apply mask (for vis images, mask_color=white)
  device, masked2 = pcv.apply_mask(masked, ab_fill, 'white', device, args.debug)
  
  # Identify objects
  device, id_objects,obj_hierarchy = pcv.find_objects(masked2, ab_fill, device, args.debug)

  # Define ROI
  device, roi1, roi_hierarchy= pcv.define_roi(img,'rectangle', device, None, 'default', args.debug,True, 30, 25,-10,-15)
  
  # Decide which objects to keep
  device,roi_objects, hierarchy3, kept_mask, obj_area = pcv.roi_objects(img,'partial',roi1,roi_hierarchy,id_objects,obj_hierarchy,device, args.debug)
  
  # Object combine kept objects
  device, obj, mask = pcv.object_composition(img, roi_objects, hierarchy3, device, args.debug)
  
############## Analysis ################  
  
  # Find shape properties, output shape image (optional)
  device, shape_header,shape_data,shape_img = pcv.analyze_object(img, args.image, obj, mask, device,args.debug,args.outdir+'/'+filename)
  
  # Shape properties relative to user boundary line (optional)
  device, boundary_header,boundary_data, boundary_img1= pcv.analyze_bound(img, args.image,obj, mask, 25, device,args.debug,args.outdir+'/'+filename)
  
  # Determine color properties: Histograms, Color Slices and Pseudocolored Images, output color analyzed images (optional)
  device, color_header,color_data,norm_slice= pcv.analyze_color(img, args.image, kept_mask, 256, device, args.debug,'all','rgb','v','img',300,args.outdir+'/'+filename)
  
  # Output shape and color data

# Median Filter
  device, soil_mblur = pcv.median_blur(soil_fill, 5, device, args.debug)
  device, soil_cnt = pcv.median_blur(soil_fill, 5, device, args.debug)
  
  # Apply mask (for vis images, mask_color=white)
  device, masked2 = pcv.apply_mask(soil_masked, soil_cnt, 'white', device, args.debug)
  
  # Identify objects
  device, id_objects,obj_hierarchy = pcv.find_objects(masked2, soil_cnt, device, args.debug)

  # Define ROI
  device, roi1, roi_hierarchy= pcv.define_roi(img,'circle', device, None, 'default', args.debug,True, 0,50,-1100,-1100)
  
  # Decide which objects to keep
  device,roi_objects, hierarchy3, kept_mask, obj_area = pcv.roi_objects(img,'partial',roi1,roi_hierarchy,id_objects,obj_hierarchy,device, args.debug)
  
  # Object combine kept objects
  device, obj, mask = pcv.object_composition(img, roi_objects, hierarchy3, device, args.debug)
  
############## Analysis ################  
  
  # Find shape properties, output shape image (optional)
  device, shape_header,shape_data,shape_img = pcv.analyze_object(img, args.image, obj, mask, device,args.debug,args.outdir+'/'+filename)
  
  # Determine color properties: Histograms, Color Slices and Pseudocolored Images, output color analyzed images (optional)
  device, color_header,color_data,norm_slice= pcv.analyze_color(img, args.image, kept_mask, 256, device, args.debug,'all','rgb','v','img',300,args.outdir+'/'+filename)
  
  # Output shape and color data
  pcv.print_results(args.image, shape_header, shape_data)
  pcv.print_results(args.image, color_header, color_data)

# Median Filter
  device, s_mblur = pcv.median_blur(fmax_thresh, 5, device, args.debug)
  device, s_cnt = pcv.median_blur(fmax_thresh, 5, device, args.debug)
  
  # Fill small objects
  device, s_fill = pcv.fill(s_mblur, s_cnt, 110, device, args.debug)
  device, sfill_cnt = pcv.fill(s_mblur, s_cnt, 110, device, args.debug)
  
  # Identify objects
  device, id_objects,obj_hierarchy = pcv.find_objects(mask, sfill_cnt, device, args.debug)
  
  # Define ROI
  device, roi1, roi_hierarchy= pcv.define_roi(mask,'circle', device, None, 'default', args.debug,True, 0,0,-50,-50)
  
  # Decide which objects to keep
  device,roi_objects, hierarchy3, kept_mask, obj_area = pcv.roi_objects(mask,'partial',roi1,roi_hierarchy,id_objects,obj_hierarchy,device, args.debug)
  
  # Object combine kept objects
  device, obj, masked = pcv.object_composition(mask, roi_objects, hierarchy3, device, args.debug)
  
################ Analysis ################  
  
  # Find shape properties, output shape image (optional)
  device, shape_header,shape_data,shape_img = pcv.analyze_object(mask, args.fmax, obj, masked, device,args.debug, args.outdir+'/'+filename)
  
  # Fluorescence Measurement (read in 16-bit images)
  fdark=cv2.imread(args.fdark, -1)
  fmin=cv2.imread(args.fmin, -1)
  fmax=cv2.imread(args.fmax, -1)
  
  device, fvfm_header, fvfm_data=pcv.fluor_fvfm(fdark,fmin,fmax,kept_mask, device, args.outdir+'/'+filename, 1000, args.debug)

device, masked2_a = pcv.rgb2gray_lab(masked2, 'a', device, args.debug)
  device, masked2_b = pcv.rgb2gray_lab(masked2, 'b', device, args.debug)
  
  # Threshold the green-magenta and blue images
  device, masked2a_thresh = pcv.binary_threshold(masked2_a, 127, 255, 'dark', device, args.debug)
  device, masked2b_thresh = pcv.binary_threshold(masked2_b, 128, 255, 'light', device, args.debug)
  device, ab_fill = pcv.logical_or(masked2a_thresh, masked2b_thresh, device, args.debug)
  
  # Identify objects
  device, id_objects,obj_hierarchy = pcv.find_objects(masked2, ab_fill, device, args.debug)
  
  # Define ROI
  device, roi1, roi_hierarchy= pcv.define_roi(masked2,'rectangle', device, None, 'default', args.debug,True, 550, 0,-600,-915)
  
  # Decide which objects to keep
  device,roi_objects, hierarchy3, kept_mask, obj_area = pcv.roi_objects(img,'partial',roi1,roi_hierarchy,id_objects,obj_hierarchy,device, args.debug)
  
  # Object combine kept objects
  device, obj, mask = pcv.object_composition(img, roi_objects, hierarchy3, device, args.debug)
  
  ############## VIS Analysis ################
  
  outfile=False
  if args.writeimg==True:
    outfile=args.outdir+"/"+filename
  
  # Find shape properties, output shape image (optional)
  device, shape_header,shape_data,shape_img = pcv.analyze_object(img, args.image, obj, mask, device,args.debug,outfile)
  
  # Shape properties relative to user boundary line (optional)
  device, boundary_header,boundary_data, boundary_img1= pcv.analyze_bound(img, args.image,obj, mask, 885, device,args.debug,outfile)