How to use the pyautogui.mouseUp function in PyAutoGUI

keyboard.add_hotkey('Ctrl + 2', lambda: pyautogui.mouseUp() or winsound.Beep(1000, duration=50))

def click_up(self, button=MouseButton.LEFT, **kwargs):
        if ("force" in kwargs and kwargs["force"] is True) or self.game_is_focused:
            pyautogui.mouseUp(button=mouse_button_mapping[button.name])

def mouse_up(self, button='left'):
        '''Releases specified mouse button'''
        ag.mouseUp(button=button)

while True:
        _, img = cam.read()
        img = cv2.flip(img, 1)
        imgHSV = cv2.cvtColor(img, cv2.COLOR_BGR2HSV)
        mask = cv2.inRange(imgHSV, lower, upper)
        blur = cv2.medianBlur(mask, 15)
        blur = cv2.GaussianBlur(blur , (5,5), 0)
        thresh = cv2.threshold(blur,0,255,cv2.THRESH_BINARY+cv2.THRESH_OTSU)[1]
        contours = cv2.findContours(thresh.copy(), cv2.RETR_TREE, cv2.CHAIN_APPROX_NONE)[1]
        mposx, mposy = gui.position()

        #print(flags)
        if len(contours) >= 2:
            old_center[0] = center[0]
            if is_mouse_down:
                Thread(target=gui.mouseUp, args=()).start()
                is_mouse_down = False
            # if left click was done
            if flags[1] == True:
                is_mouse_down = False

                # do a right click if the fingers were together for 10-20 frames and then released
                if finger_frame_count[1] >= 10 and finger_frame_count[1] <= 20:
                    gui.rightClick()
                # else if fingers were together for less than 10 frames then do a left click
                elif finger_frame_count[1] < 10:
                    gui.click()
                finger_frame_count[1] = 0
            finger_frame_count[2] += 1

            c1, c2 = top(contours, cv2.contourArea, 2)

def drag_mouse( self, should_drag ):
		if( self.should_drag != should_drag ):
			if( should_drag == True ):
				print( "Start dragging!" )			
				mouseDown()
			else:
				print( "Stopped dragging!" )			
				mouseUp()
				
		self.should_drag = should_drag

# do a left click and hold if the fingers were together for more than 20 frames
                if finger_frame_count[1] > 20:
                    if not is_mouse_down:
                        gui.mouseDown()
                        is_mouse_down = True
                    if np.any(abs(center[1] - old_center[1]) > 5):
                        Thread(target=gui.moveTo, args=(mposx+(center[1][0]-old_center[1][0])*sx, mposy + (center[1][1]-old_center[1][1])*sy, \
                            0.1, gui.easeInOutQuad)).start()     
                flags = [False, True, False]
            else:
                flags = [True, False, False]

        else:
            if is_mouse_down:
                Thread(target=gui.mouseUp, args=()).start()
                thread.start_new_thread(gui.mouseUp, ())
                is_mouse_down = False
            flags = [True, False, False]

        cv2.imshow("Virtual Mouse", img)
        if cv2.waitKey(1) == ord('q'):
            break

    cam.release()
    cv2.destroyAllWindows()
    gui.mouseUp()

if t.data == "delay":
        sec = do(t.children[0])
        pyautogui.PAUSE = sec
        return

    if t.data == "drag":
        btn = do(t.children[0])
        cs = []
        for c in t.children[1:]:
            cs.append(do(c))

        # pyautogui.drag(*cs, button=btn)
        # hack, because the cursor keeps moving...?
        pyautogui.mouseDown(button=btn)
        pyautogui.moveTo(cs[0], cs[1])
        pyautogui.mouseUp(button=btn)
        return

    if t.data == "coord":
        name = do(t.children[0])
        t = do(t.children[1])

        pnt = coords[name][screen]
        x, y = pnt[0], pnt[1]

        pyautogui.moveTo(x, y, t, pyautogui.easeOutQuad)
        return

    if t.data == "coord_off":
        name = do(t.children[0])
        x = do(t.children[1])
        y = do(t.children[2])

def mouseUpAction( self, button='left' ):
        print( "----------> RELEASING MOUSE " + button )    
        pyautogui.mouseUp( button=button )